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Plant Dis 94:515–520Ĭarling DE (1996) Grouping in Rhizoctonia solani by hyphal anastomosis reaction. IEEE Trans Automat Contr 19:716–723īartz FE, Cubeta MA, Toda T, Naito S, Ivors KL (2010) An in planta method for assessing the role of basidiospores in Rhizoctonia foliar disease of tomato. solani isolates based on the combined data from the rDNA-ITS, rDNA-IGS1 regions, and tef-1α gene.Īkaike H (1974) A new look at the statistical model identification. This study is the first report on the phylogenetic analysis of R.

New subgroup AG-3 TM (tomato type) is proposed. These results indicate that the 10 isolates from tomato and soil represent a new subgroup distinct from AG-3 PT and TB. A phylogenetic tree based on these three loci indicated that the 10 isolates form a new clade separated from AG-3 PT and TB populations. The 10 AG-3 isolates were genetically closer to AG-3 PT than to AG-3 TB on the basis of rDNA-ITS and tef-1α. In the sequence comparisons of these loci, all 10 AG-3 isolates shared high sequence similarity (99.4–100% for rDNA-ITS, 99.1–100% for rDNA-IGS1, and 98.2–100% for tef-1α) to each other, but a comparatively low similarity (95.4–98.8% for rDNA-ITS, 94.7–98.3% for rDNA-IGS1, and 93.5–97.2% for tef-1α) to AG-3 PT and AG-3 TB isolates. Three loci of 16 AG-3 PT (potato type) and eight AG-3 TB (tobacco type) isolates were also sequenced to evaluate genetic relationships with these 10 isolates. Ten Rhizoctonia solani AG-3 isolates obtained from blighted tomato leaves and from soil (one isolate, collected from Australia) were examined for sequence variations in the rDNA-ITS, rDNA-IGS1 regions, and tef-1α gene.